Fig 2: Real-time PCR analysis of (A) OAT1 mRNA, and (B) OAT3 mRNA expression in kidney tissues of expression levels were normalized to the reference gene B2m using the comparative Ct method (2-?? Ct). Results were presented as mean ± SE (n = 6). * p = 0.05 relative to the control group; # p = 0.05 relative to cisplatin group; ? p = 0.05 relative to ellagic acid nano (1 mg/kg) group.

Fig 3: Effects of hesperetin (HSE) on serum parameters and protein levels in mice with hyperuricemia induced by potassium oxonate. (A) Uric acid (UA) (B) Blood urea nitrogen (BUN) (C) Serum creatinine (CRE). Protein expression of OAT1 (E), OAT3 (F), OCT1 (G), OCT2 (H), and ß-tubulin were determined via Western blotting quantified with ImageJ. The dose of allopurinol was 10 mg/kg in vivo experiments. These data were from three separate experiments and expressed as the mean ± SEM. # p < 0.05 and ## p < 0.01 versus the normal group; *p < 0.05 and **p < 0.01 versus the model group. HSE, hesperetin; UA, uric acid; BUN, blood urea nitrogen; CRE, serum creatinine; PO, potassium oxonate; XOD, xanthine oxidase; OAT1, organic anion transporter 1; OAT3, organic anion transporter 3; OCT1, organic cationic transporters 1; OCT2, organic cationic transporters 2.